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immunocult human th1 differentiation supplement  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc immunocult human th1 differentiation supplement
    Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for <t>Th1</t> cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
    Immunocult Human Th1 Differentiation Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunocult+human+th1+differentiation+supplement/pmc08201873-82-0-5?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    immunocult human th1 differentiation supplement - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells"

    Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

    Journal: Respiratory Research

    doi: 10.1186/s12931-021-01757-1

    Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
    Figure Legend Snippet: Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.

    Techniques Used: Knockdown

    Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant
    Figure Legend Snippet: Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Techniques Used: Expressing, Staining, Real-time Polymerase Chain Reaction

    Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant
    Figure Legend Snippet: Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Techniques Used: Knockdown, Expressing, Fluorescence, Negative Control, Staining



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    immunocult human th1 differentiation supplement  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc immunocult human th1 differentiation supplement
    Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for <t>Th1</t> cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.
    Immunocult Human Th1 Differentiation Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunocult+human+th1+differentiation+supplement/pmc08201873-82-0-5?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    immunocult human th1 differentiation supplement - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.

    Journal: Respiratory Research

    Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

    doi: 10.1186/s12931-021-01757-1

    Figure Lengend Snippet: Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45 + and a CD45 − cell population. Within the CD45 + cell population, gate for CD4 T cells (CD3 + CD4 + ). Within the CD4 T cells, gate for Th1 cells (T-bet + ) and Th2 cells (GATA-3 + ). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3 + ) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.

    Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

    Techniques: Knockdown

    Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Journal: Respiratory Research

    Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

    doi: 10.1186/s12931-021-01757-1

    Figure Lengend Snippet: Expression level of SERPINB10 in human Th cells. a flow cytometric plots for Th1 or Th2 polarized CD4 T cells from patients with asthma after intracellular staining for IFN-γ or IL-4. b the transcript level of IFNG, T-bet, IL-4 and GATA-3 in polarized Th1 and Th2 cells was measured using quantitative PCR. c cytokine levels in culture supernatant of polarized Th1 or Th2 cells were measured by LEGENDplex human Th cytokine panel. d SERPINB10 transcript level (fold change over naïve CD4 T cells) in Th1 or Th2 cells after no stimulation or stimulation with anti-CD3 or cytokine (IFN-γ for Th1 and IL-4 for Th2). n = 16 patient samples. Data were means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

    Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction

    Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Journal: Respiratory Research

    Article Title: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells

    doi: 10.1186/s12931-021-01757-1

    Figure Lengend Snippet: Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP) + percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ + or IL-4 + polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ + or IL-4 + polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant

    Article Snippet: ImmunoCult Human Th1 Differentiation Supplement (StemCell Technologies) and ImmunoCult Human Th2 Differentiation Supplement (StemCell Technologies) were added to direct the differentiation of Th1 and Th2 cells, respectively.

    Techniques: Knockdown, Expressing, Fluorescence, Negative Control, Staining